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Schraidt O., Marlovits TC (2011)

Three-dimensional model of Salmonella’s Needle Complex at Subnanometer Resolution.

Science 331:1192-95

Primary data (negatively stained) of end-view outer ring particles of the needle complex

(used to determine 15-fold symmetry in the “outer” ring (secretin family)

Primary data (cryo EM) of end-view needle complex particles

(used to determine the 3-fold symmetry of entire needle complexes, composed of a 24-fold “inner” (larger diameter) and a 15-fold “outer” (smaller diameter) ring)